Chromogen

"Red wine, unlike white, contains a highly-pigmented substance known as chromogen," explained Dr. Wolff. ❋ Unknown (2009)

However much we may learn of chlorophyl, chromogen, and colour-cells – the pigments of nature that are made from earth and rain, air and sun, somewhere in the dark habitation of the roots and the airy galleries of the leaves – we do not know why the same ingredients should clothe one petal with flame and another with blue. ❋ Unknown (1917)

This was followed by a 5-min incubation with the substrate-chromogen, 3,3′-diaminobenzidine (Vector SK-4100). ❋ Jeran K. Stratford Et Al. (2010)

Double immunostainings with anti-LMP2/anti-Olig2 and with anti-PA28-α/anti-Olig2 were performed following the previous procedure but employing as second chromogen Perma\Blue AP Chromogen (Diagnostic Biosystems, Pleasonton, CA, USA) for alkaline phosphatase. ❋ Michele Mishto Et Al. (2010)

After washing, the HRP (horseradish peroxidase) conjugated polymer complex was visualized by incubation for ~5 min with AEC (3-amino-9-ethylcarbazole) substrate-chromogen; sections were then counterstained with hematoxylin for 30 seconds, washed and mounted. ❋ Unknown (2010)

AEC substrate-chromogen was purchased from Dako and cocktail protease and phosphatase solutions for protein harvest from Roche ❋ Veronica Ulici Et Al. (2010)

Finally, antibody binding was visualised using DAB as the chromogen and the sections mounted in Mowiol for observation under a Nikon 90i microscope. ❋ Hortensia Sanchez-Calderon Et Al. (2010)

The cE.ISA was provided and performed by VLA staff according to the O.I.E. Manual of Standards for Diagnostic Tests and Vaccines using the 16M Melitensis strain as antigen and OPD as the chromogen, stopped with Citric acid. ❋ Francesca Scolamacchia Et Al. (2010)

Diaminobenzidine (0. 05\%; Sigma) with nickel chloride (0. 04\%) was used as chromogen, with reactions sustained for 10 minutes at room temperature. ❋ Unknown (2009)

Diaminobenzidine (DAB, Sigma) was used as a chromogen at 0.25 mg / ml in TBS with 0. 01\% H ❋ Unknown (2009)

After three washes with TBS, the samples were incubated with streptavidin-biotin peroxidase (LSAB kit; Dako) for 10 min using diaminobenzidine tetrahydrochlorideas the chromogen. ❋ Unknown (2009)

Immunostaining was performed with the Lab Vision Autostainer (Lab Vision, Fremont, USA) using a biotin-streptavidin-peroxidase detection system with diaminobenzidine as a chromogen ❋ Unknown (2009)

Peroxidase activity was detected by using 3,3′ diaminobenzidine (DAB) as chromogen with hydrogen peroxide as substrate. ❋ Deepti Joshi Et Al. (2009)

(DAKO, Carpinteria, CA) with diaminobenzidine tetrahydrochloride (3,3′-diaminobenzidine) as the chromogen. ❋ Payel Bhanja Et Al. (2009)