This new method, which is based on citraconic acid, has not been previously used in brain tissue although it has been employed in various other tissues such as tonsil, ovary, skin, lymph node, stomach, breast, colon, lung and thymus. ❋ Unknown (2008)
This new method, which is based on the use of citraconic acid, has not been previously utilized in brain tissue although it has been employed in various other tissues such as tonsil, ovary, skin, lymph node, stomach, breast, colon, lung and thymus. ❋ Unknown (2008)
High power view of some CB-ir neurons in the human mediodorsal thalamic nucleus without the employ of citraconic anhydride solution. ❋ Unknown (2008)
Briefly, after a 0.1 M PB and 0. 2\% Triton X-100 rinse cycle of three ten minutes rinses, the slices were immersed in a Petri dishes (60 mm×15 mm) sealed with Parafilm® and shaken for 45 minutes in a 0. 05\% citraconic anhydride solution (volume for each Petri dish: 3 ml; pH 7.4) at ❋ Unknown (2008)
Once the optimal conditions to carry out immunohistochemical procedures were established (45 minutes in a 0. 05\% citraconic anhydride solution, pH 7.4, at 95°C), formalin fixed brain tissue sections were subjected to the AR method, obtained a similar intensity of immunostaining compared to a protocol widely used in immunohistochemical studies ❋ Unknown (2008)
Bright field photomicrographs in high magnification of ENK fibers without citraconic anhydride solution in the caudate nucleus. ❋ Unknown (2008)
This group concluded that the AR method based on citraconic anhydride allowed them to carry out optimal immunostaining procedures in comparison of the existing AR protocols, so the extent and the intensity of immunostainings is enhanced ❋ Unknown (2008)
Moreover, this new AR method provides some advantages, for instance the reliability, the possibility to establish as a universal AR method, and the possibility to process the slices in one batch have employed the citraconic anhydride in several tissues, as far as we know nobody has tested this AR method in human brain sections for immunohistochemical free-floating studies. ❋ Unknown (2008)
A, Interneuron immunohistochemistry in the MD. High power view of some GAD-ir neurons in the mediodorsal thalamic nucleus without the use of citraconic anhydride solution. ❋ Unknown (2008)
This AR method, which utilizes a citraconic anhydride solution, has previously been shown to be superior to conventional AR methods in various tissues (Citrate buffer and Tris-HCL+5\% urea) ❋ Unknown (2008)
High power view (10× and 20× magnification respectively) of TH-ir neurons in the substantia nigra employing the citraconic anhydride solution. ❋ Unknown (2008)
In order to maintain the same conditions for all the slices used in the experiment, the tissue does not be immersed in the citraconic anhydride solution until the water bath reach the 95°C. ❋ Unknown (2008)
