Skerra 2000 A novel type of receptor protein, based on the lipocalin scaffold, with specificity for digoxigenin. ❋ Unknown (2010)
Sections were incubated overnight at 70°C with 1 µg/ml of the digoxigenin-labeled probes, and binding detected by overnight incubation with alkaline phosphatase-conjugated anti-digoxigenin antibody (1: 3500, Roche), which was visualised with NBT (Nitro blue tetrazolium chloride)/BCIP (5-Bromo-4-chloro-3-indolyl phosphate, toluidine salt; 1: 50, Roche) or Fast Red (Roche) for fluorescence. ❋ Hortensia Sanchez-Calderon Et Al. (2010)
For immunohistological detection of digoxigenin (DIG), the sections were incubated overnight at room temperature with anti-DIG conjugated to alkaline phosphatase (Roche Diagnostic) diluted 1: 2000 in PBST containing 0. 2\% blocking agent (Roche Diagnostic). ❋ Unknown (2009)
For single-color fluorescent in situ hybridizations (FISH) using a digoxigenin-conjugated RNA probe and tyramide signal amplification (TSA-Plus kit; PerkinElmer), the above procedure was modified as follows. ❋ Unknown (2009)
For dual-color FISH with fluorescein - and digoxigenin-conjugated RNA probes, after the blocking step, embryos were incubated in POD-conjugated anti-fluorescein antibody (Roche) (1: 750) at RT overnight, followed by eight washes with MOPS wash buffer. ❋ Unknown (2009)
In Situ Cell Death Detection kit-AP (Roche, Germany) according to a previously described protocol Clones of the in situ markers were obtained from various labs (mentioned in the Acknowledgement section) and digoxigenin-labeled antisense RNA probes were generated with T7 or T3 RNA polymerases (Roche) according to the orientation of cDNA inserts. ❋ Unknown (2009)
AS RNA, antisense RNA; ChIP, chromatin immunoprecipitation; DIG, digoxigenin; IP, inositol polyphosphate; ncRNA, noncoding RNA; Pi, inorganic phosphate ❋ Unknown (2009)
Total RNA was isolated from the wt, Δ pho4, or Δpho85 cells grown under high (H) - or low (L) - Pi conditions, subjected to northern blot analysis, and probed with a digoxigenin (DIG) - labeled probe for each gene as designated. ❋ Unknown (2009)
DNA probes were prepared by PCR using digoxigenin (DIG) - PCR labeling mix (Roche). ❋ Unknown (2009)
Apoptosis was confirmed by DNA fragmenatation, western blot and terminal transferase-mediated dUTP-digoxigenin-end labeling (TUNEL) assay. ❋ Gowhar Shafi (2009)
Apoptotic cells were detected in situ by performing TUNEL (TdT-mediated digoxigenin labeled dUTP nick end labeling) staining. ❋ Payel Bhanja Et Al. (2009)
Probes specific for pHJO or pBeloBAC11 fragments were generated by random labelling with digoxigenin of sonicated purified BAC DNA. ❋ Felix Wussow Et Al. (2009)
A digoxigenin (DIG) labeled cDNA probe against putative opsin RNA of ❋ Hiroshi Yamashita Et Al. (2009)
(a) Northern dot blot analysis using a digoxigenin-labeled opsin cDNA probe for a dinoflagellate ❋ Hiroshi Yamashita Et Al. (2009)
Hybridization and detection procedures using colorimetric alkaline phosphatase-conjugated digoxigenin antibodies were carried out as described previously [ ❋ Unknown (2009)
The gels were then denatured, blotted onto charged nylon membranes (Amersham Biosciences), and hybridized with a digoxigenin (DIG) - labeled telomeric DNA probe ( ❋ Unknown (2008)
