Eluted

The four proteins that bound and eluted to the greatest extent were expressed in Escherichia coli as C-terminal fusion proteins of maltose binding protein (MBP), as the solubilities of the free proteins were too low to permit full characterization. ❋ Unknown (2009)

During the column chromatography of aequorin, we found a trace of protein that showed green fluorescence, which eluted sooner than aequorin. ❋ Unknown (2009)

SELEX (systematic evolution of ligands by exponential enrichment) is a method to generate DNA … which are then eluted from the target molecule and amplified by PCR. … (both of which are used in Macugen, an FDA-approved aptamer), … The development of aptamer probes for molecular signatures on the cancer cell … ❋ Unknown (2010)

¬† Without the total control that Dave eluted to the final product might have been convoluted and certainly delayed. ❋ Unknown (2007)

Fraction I, nor in the high salt eluted material (denoted Fraction II). ❋ Unknown (2005)

Alcohol (isopropanol) precipitation concentrates the eluted plasmid DNA, which is collected by centrifugation, washed, and redissolved in buffer. ❋ Unknown (2005)

Unwanted materials, including RNA, DNA, and proteins, are thoroughly washed from the column before the plasmid DNA is eluted using a high salt buffer. ❋ Unknown (2005)

"Over the near-term, we believe that the shares will continue to face pressure as it deals with the FDA warning letters, tries to maintain drug-eluted stent share and works to close and integrate the Guidant acquisition," wrote analyst Glenn Novarro in a report today. ❋ Unknown (2006)

The more rapidly moving zone is d-lysergic acid N,N-diethylamide which is eluted with about 3000 ml. of the same solvent as above, the course of the elution being followed by watching the downward movement of the more rapidly moving blue fluorescing zone. ❋ Unknown (2006)

Using the reverse phase system, this elution sequence is broadly in accordance with their polarities: aloe-emodin polar, and physcion, least polar is eluted last. ❋ Unknown (1991)

Anthraquinones were consistently eluted in the sequence, aloe-emodin, rhein, emodin, chrysophanol and physcion. ❋ Unknown (1991)

Beta-glucosidases split the glucosides (the bacteria use the sugar), and the nitrites and sulphur compounds and degraded; other molecules may be eluted. ❋ Unknown (1979)

The newly discovered peptide eluted between insulin and proinsulin on Sephadex G50 gel filtration, in contrast with HG which eluted after insulin (Fig. 12). ❋ Unknown (1977)

Clarified cell lysate was loaded onto a Nickel Chelating Sepharose Fast Flow column (GE healthcare), the His-tagged SSADH protein was eluted in 150 mM NaCl, 250 mM imidazole, 5 mM β-mercaptoethanol, 50 mM Tris (pH 8.0). ❋ Christopher G. Langendorf Et Al. (2010)

Another possibility was that the gentamicin eluted too rapidly to add efficacy to the systemic antibiotics patients were given preoperatively, they speculated. ❋ Unknown (2010)

Material bound to washed beads was eluted, boiled, separated, and probed with rabbit antibodies against Cav1. 2 (Alomone) or V5 (Sigma). ❋ Ting-Ting Hong Et Al. (2010)

Reverse phase HPLC was carried out using a C4 Phenomenex Jupiter (150×4. 6 mm) and eluted with a gradient of acetonitrile and trifluoroacetic acid (TFA) 0. 085% versus water and 0. 1% TFA: from 5 to 35% over 5 min, from 35 to 55% over 20 min, from 55 to 95% over 2 min, at a flow rate of 0.6 mL/min. ❋ Diane Latawiec Et Al. (2010)

After washes, bound surface proteins were eluted and boiled, separated on NuPage gels (Invitrogen), and probed with rabbit anti-Cav1. 2 antibody (Alomone) and mouse anti-NCX1 antibody (Abcam). ❋ Ting-Ting Hong Et Al. (2010)

The dimer eluted at approximately 13.5 mL (PD) and was separated from any remaining soluble aggregates that eluted in the void volume (PA). ❋ Carlene S. Starck Et Al. (2010)

The supernatant was taken and passed through Ni-NTA agarose column (Qiagen) and washed thoroughly with wash buffer (20 mM of Tris, pH 7.9 containing 30 mM of imidazole and 0. 5M sodium chloride) before eluted with wash buffer containing 0.5 M of imidazole. ❋ Janarthanan Krishnamoorthy Et Al. (2010)