Hybridomas

Köhler and Milstein called these hybrid cells hybridomas, and as all cells in a given hybridoma come from one single hybrid cell, the antibodies made are monoclonal. ❋ Unknown (1984)

These monoclonal antibodies are produced by hybridomas. ❋ Unknown (2010)

The mouse spleen and lymph node lymphocytes were fused to firm hybridomas. ❋ Unknown (2010)

A mixture of un-transfected and mouse BAFF-R-expressing Y3 rat myeloma cells was used to screen supernatants of individual hybridomas generated as described in ❋ Unknown (2009)

In total, eleven hybridomas producing anti-BAFF-R mAbs were obtained and of these, five were of rat IgG2a and 6 of IgG2b isotype. ❋ Unknown (2009)

Injection of the antigen into mice, generation of hybridomas, clone screening, and clone isolation were performed in the Mount Sinai Hybridoma Shared Research Facility. ❋ Unknown (2009)

From what I can understand, the prior art was human-mouse hybridomas and the basic advance was a method of producing human-human hybridomas. ❋ Unknown (2008)

So far as I can tell, it was well known in the prior art that problems associated with the therapeutic use of mAbs derived from human-mouse hybridomas arose because of they yielded mouse antibodies, so I can't image that the notion of using human-human hybridomas was innovative in itself. ❋ Unknown (2008)