C. neoformans as dominant selectable markers (such as hygromycin B), we used the ❋ Sven D. Willger Et Al. (2009)
SureSilencing shRNA plasmids against HSP90α and β isoforms containing the hygromycin resistance gene were obtained from SABiosciences (Frederick, MD). ❋ Daniela Trisciuoglio Et Al. (2010)
E. coli and 25 µg/ml for M. smegmatis; and hygromycin B, 100 µg/ml for oppA (Rv1280c) of M. tuberculosis was amplified from cosmid MTCY50 using the primer pair ❋ Arunava Dasgupta Et Al. (2010)
Complementation of oppAMTB in the OppA-KO oppA was achieved by constructing an integrating vector containing a hygromycin-resistance cassette along with a positive-selection L5 integrase cassette. oppA of M. tuberculosis (wild type or variants) was first cloned in pOLYG ❋ Arunava Dasgupta Et Al. (2010)
Complementation was therefore carried out with oppDA by constructing an integrating vector containing a hygromycin-resistance cassette along with a positive-selection L5 integrase cassette. ❋ Arunava Dasgupta Et Al. (2010)
[10] (DsRed) were split in the middle and fused to each other with intervening drug selection markers (hygromycin - or neomycin-resistance gene) franked by loxP sites. ❋ Munehiro Uemura Et Al. (2010)
The white, kanamycin and hygromycin-sensitive, sucrose-resistant colonies were identified, analyzed by PCR and candidate ❋ Arunava Dasgupta Et Al. (2010)
Hygromycin was then shuttled from the linearized hygromycin marker (Clontech) to make pIRES-hyg-pcDNA3. 1z. ❋ Hongyu Ying Et Al. (2010)
Cell lines with stably integrated cDNAs were selected with hygromycin (100 µg/ml) (Mediatech). ❋ Miguel Aste-Amézaga Et Al. (2010)
Fortunately, RGC5 cells are sensitive to hygromycin. ❋ Hongyu Ying Et Al. (2010)
The growth medium was changed to selection medium containing 100 µg/ml of hygromycin and the selection continued for about 2 weeks until medium sized colonies grew out. ❋ Hongyu Ying Et Al. (2010)
Chr. 17, chromosome 17; hyg, hygromycin resistance gene; neoSD, neo splice donor; SA neo, splice acceptor neo; puro, puromycin resistance gene. ❋ Deniz Simsek (2010)
ES - and pCEP4-B1. 8-NC1ES - transfected HEK-293 cells were selected in DCM with 150 µg / ml hygromycin B (Invitrogen). ❋ Unknown (2009)
The cells were maintained in the same medium as above but supplemented with 0.2 mg/ml G418 and 0.1 mg/ml hygromycin B. ❋ Luke W. Meredith Et Al. (2009)
The regions used as porbes are indicated by solid lines with square ends while the expected bands are indicated by broken lines (with sizes indicated). hyg, hygromycin resistance gene from Streptomyces hygroscopicus doi: 10.1371/journal. pone.0006306.g004 ❋ Alistair K. Brown Et Al. (2009)
For M. sm, hygromycin and Trimethoprim (Trim) were used at a concentration of 50 µg/ml. ❋ Garima Khare Et Al. (2009)
Transduced cells were selected by incubation with hygromycin B (Sigma-Aldrich, St. Louis, MO) at a concentration of 500 µg/ml for 14 days. ❋ Christopher A. Hamm Et Al. (2009)
