Hyh

Sperm from wild type and SP hyh mice were collected from the cauda epididymidis and incubated under capacitation conditions for 1 h. ❋ Unknown (2009)

Wild type αSNAP rescues acrosomal exocytosis in sperm from hyh mice ❋ Unknown (2009)

Acrosome reaction experiments indicate that the basic problem in sperm from hyh mice is a reduced capacity to undergo exocytosis upon stimulation with progesterone and even with a calcium ionophore. ❋ Unknown (2009)

Black bars (mean±SEM, N = 7) refer to the relative amount of each protein in hyh samples compared to wt (gray bars). * p doi: 10.1371 / journal. pone.0004963.g006 ❋ Unknown (2009)

Bronson RT, Lane PW (1990) Hydrocephalus with hop gait (hyh): a new mutation on chromosome 7 in the mouse. ❋ Unknown (2009)

Brains from wild type (normal phenotype), and SP hyh and RP hyh mice from the same litter (70 day old) were fixed with Bouin solution by vascular perfusion and coronally sectioned at the level of the optic chiasm. ❋ Unknown (2009)

The above results indicate that the acrosomal exocytosis defect in hyh mice was not due to a diminished amount of αSNAP. ❋ Unknown (2009)

Chae TH, Kim S, Marz KE, Hanson PI, Walsh CA (2004) The hyh mutation uncovers roles for alpha-SNAP in apical protein localization and control of neural cell fate. ❋ Unknown (2009)

Following capacitation, sperm from wild type and hyh mice were diluted to 2 or 4×10 ❋ Unknown (2009)

Sperm characteristics in wild type and SP hyh (hyh) mice. ❋ Unknown (2009)

Testes were rapidly removed from hyh and wild type mice and fixed in Bouin fixative solution for 48 h at room temperature. ❋ Unknown (2009)

Even when fertilization was assessed in vitro, sperm from hyh mice behave poorly as compared with sperm from wild type mice. ❋ Unknown (2009)

Sperm from SP hyh mice have a deficient acrosomal reaction. ❋ Unknown (2009)

In vitro fertilization (IVF) assays using sperm from wild type and SP hyh mice. doi: 10.1371 / journal. pone.0004963.t002 ❋ Unknown (2009)

Sperm suspensions from hyh and wild type mice were fixed in 2\% paraformaldehyde. ❋ Unknown (2009)

Black bars (mean±SEM, N = 3 or 4) refer to the relative amount of each protein in hyh samples compared to wt. * p doi: 10.1371 / journal. pone.0004963.g004 ❋ Unknown (2009)

We have previously described that hyh mice present a heterogenous neuropathological and clinical phenotype ❋ Unknown (2009)

Consistent with an hypomorphic phenotype, the testes and epididymides of hyh mice had low amounts of the mutated protein. ❋ Unknown (2009)

In particular, hydrocephalus with hop gait (hyh) is a recessive mouse disease that arose spontaneously in the C57BL / 10J strain ❋ Unknown (2009)

Sperm from two wild type male mice and from at least two hyh male mice were obtained as explained above. ❋ Unknown (2009)