Immunoblot

Word IMMUNOBLOT
Character 10
Hyphenation N/A
Pronunciations N/A

Definitions and meanings of "Immunoblot"

What do we mean by immunoblot?

A laboratory procedure, such as Western blot analysis, in which proteins that have been separated by electrophoresis are transferred onto nitrocellulose sheets and are identified by their reaction with labeled antibodies. noun

A blot used in western blot analysis noun

To perform such an analysis verb

Here you will find one or more explanations in English for the word immunoblot. Define immunoblot, immunoblot synonyms, immunoblot pronunciation, immunoblot translation, English dictionary definition of immunoblot.

Synonyms and Antonyms for Immunoblot

  • Synonyms for immunoblot
  • Immunoblot synonyms not found!!!
  • Antonyms for immunoblot
  • Immunoblot antonyms not found!

The word "immunoblot" in example sentences

GM immunoblot showed an IgE-binding 14 kDa band that was totally inhibited after serum pre-incubation with GM. ❋ Steve Carper (2007)

Screen with FAST-ELISA and confirm with immunoblot. ❋ Unknown (2008)

A recombinant immunoblot assay (RIBA) that identifies antibodies which react with individual HCV antigens is often used as a supplemental test for confirmation of a positive EIA result. ❋ Unknown (2000)

Huh7 cells were transfected with pCI-vTK and G418-resistant Huh cellular clones (cl) (1-6) were evaluated for vTK expression (~43 KDa, arrowheads) by immunoblot analysis. ❋ Donna N. Douglas Et Al. (2010)

Non-adherent HCT116 and HT29 colorectal carcinoma cells expressing CXCL12 exhibited enhanced anoikis sensitivity by propidium iodide staining, caspase activity assays, and immunoblot compared to GFP control cells. ❋ PLoS ONE (2010)

(B) Expression levels of SATB1 and β-catenin in HEK 293T, Jurkat, and SW480 cells were compared by immunoblot analysis using respective antibodies. ❋ Dimple Notani Et Al. (2010)

Stabilization of β-catenin upon differentiation was monitored by immunoblot analysis of nuclear extracts from the two subtypes of T ❋ Dimple Notani Et Al. (2010)

Figure 6: Magoh acts upstream of the microcephaly-associated protein Lis1 to regulate neurogenesis. gene expression measured by quantitative PCR (left) and Lis1 protein expression measured from immunoblot analyses (right). ❋ Debra L Silver (2010)

Different band intensities corresponding to immunoblot detection of protein samples were quantified using the MCID software (MCID, Cambridge, UK). ❋ Nadia G. Innamorato Et Al. (2010)

In order to check the expression of apoptotic proteins, we ran immunoblot analysis of XIAP, Akt and pAkt molecules, which are highly upregulated in glioma cells. ❋ Venkata Ramesh Dasari Et Al. (2010)

Adhesion, examined by crystal violet staining, immunofluorescence microscopy, and immunoblot analysis indicated decreased focal adhesion signaling corresponding with loss of adhesion in cells constitutively simulated by CXCL12. ❋ PLoS ONE (2010)

Lanes 1-5, immunoblot analysis of cell extracts before immunoprecipitation (input). ❋ Daniela Di Bella (2010)

Nuclear extracts were prepared from control cells and cells polarized to T H1 and TH2 and were treated with Wnt agonist BIO or Wnt inhibitor Dkk1, followed by immunoblot analysis using anti-β-catenin (upper panel) and anti-γ-tubulin (lower panel). ❋ Dimple Notani Et Al. (2010)

Substitutions affecting respiration are in bold. (a) Fluorescence immunoblot analysis with anti-MrpL32 shows that yeast MrpL32 precursor (p) accumulates in yta10Δyta12Δ m-AAA-deficient cells (lane 2). m, mature MrpL32. ❋ Daniela Di Bella (2010)

A representative immunoblot from one animal is shown. ❋ Jessica E Rexach (2010)

Cellular expression of vTK protein was detected by immunoblot analysis essentially as described ❋ Donna N. Douglas Et Al. (2010)

Pull-down assays for RhoA or Rac1 in the presence of 5 µg/ml mitomycin-C were performed after 48 h of siRNA treatment, using an immunoblot of lysates for ❋ Shigeyuki Kanazawa Et Al. (2010)

P > 0.05. (b) Fluorescence immunoblot analysis and protein quantitation of mitochondria-encoded COX subunits Cox1p, Cox2p and Cox3p, and nuclear-encoded subunit Cox4p, in mitochondrial extracts from cells expressing AFG3L2 alone, in the absence of paraplegin. ❋ Daniela Di Bella (2010)

SDS-PAGE and immunoblot characterization of the purified recombinant protein (both from cell lysate as well as culture supernatant) revealed a doublet ranging from ~35-40 kDa. ❋ Anasua Ganguly (2010)

Cross Reference for Immunoblot

  • Immunoblot cross reference not found!

What does immunoblot mean?

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