You should have a good grounding in cardiovascular research and previous experience of relevant techniques such as in vivo cardiac phenotyping, the use of gene-modified mice, immunoblotting, RT - ❋ Unknown (2010)
Mca1 protein was detected using polyclonal anti-Mca1 antibodies and immunoblotting. ❋ Marc Erhardt Et Al. (2010)
Using real-time RT-PCR, immunoblotting, immunostaining, and siRNA-transfection, the research team analyzed the association of GPC3 expression with Wnt and other growth signaling molecules in ❋ Unknown (2010)
• General Laboratory skills including gel electrophoresis and immunoblotting. ❋ Unknown (2010)
(A) The activity of JNK with or without 10 µM SP600125 was analyzed by immunoblotting at 30 min after 100 ng/ml bFGF-stimulation in the presence of 5 µg/ml mitomycin-C. ❋ Shigeyuki Kanazawa Et Al. (2010)
Cells transfected with GFP and a plasmid encoding short hairpin RNA (shRNA) targeting cplx 2 were isolated by fluorescence-activated cell sorting and analyzed by immunoblotting. ❋ Seong J An (2010)
Validation of phosphosites identified by mass spectrometry can be done by immunoblotting in cases where phosphorylation site-specific antibodies are available. ❋ Gabriele Weintz (2010)
Immunoprecipitated pull down was verified by immunoblotting with anti-optineurin (anti-OPTN, right panel). ❋ Hongyu Ying Et Al. (2010)
Soluble and aggregated proteins were separated by centrifugation analysis and detected using polyclonal anti-Sup35C antibodies and immunoblotting. ❋ Marc Erhardt Et Al. (2010)
Samples were harvested at 72 h (before apoptosis) or 90 h (after apoptosis) for immunoblotting for ❋ Febitha Kandan-Kulangara Et Al. (2010)
(A) The activity of Akt with or without 10 µM LY294002 was analyzed by immunoblotting at 30 min after 100 ng/ml bFGF-stimulation in the presence of 5 µg/ml mitomycin-C. ❋ Shigeyuki Kanazawa Et Al. (2010)
In addition, electron microscopy and immunoblotting data indicated that these analogs induced robust autophagy in human macrophages. ❋ Susu Zughaier Et Al. (2010)
Accordingly, we could not accurately determine the molecular weight of Mca1 expressed in vivo by immunoblotting of wild type lysate with polyclonal antibodies raised against Mca1. ❋ Marc Erhardt Et Al. (2010)
Immunoprecipitated pull down was verified by immunoblotting with anti-optineurin ❋ Hongyu Ying Et Al. (2010)
This drug-induced autophagy was also confirmed by immunoblotting for microtubule-associated protein light chain 3 (LC-3), a well known marker for autophagy ❋ Susu Zughaier Et Al. (2010)
Using real-time RT-PCR, immunoblotting, immunostaining, and siRNA-transfection, the research team analyzed the association of GPC3 expression with Wnt and other growth signaling molecules in HCC. ❋ Unknown (2010)
At 72 h after transfection, cells were treated with 100 µM etoposide or with solvent control for 18 h and samples were harvested for immunoblotting for PARP, activated caspase-3, Dicer-1 and Ago-2. ❋ Febitha Kandan-Kulangara Et Al. (2010)
