As park25 flies do not produce any Parkin protein, this immunoprecipitation serves as a control for any nonspecific binding of other proteins to the anti-Parkin antiserum. ❋ Angela C. Poole Et Al. (2010)
Methods: The ribonomic approach, utilizing RNA immunoprecipitation hybridized to microarray (RIP-Chip), provides global identification of putative endogenous mRNA targets of different RBPs. ❋ Unknown (2010)
Indeed co-immunoprecipitation assay demonstrated a direct physical interaction of Sp1 with PPARγ in whole cell extracts of U937 cells. ❋ Anil K. Singh Et Al. (2010)
Lysate from flies with hsp70-GAL4 driven expression of UAS-dmfn-RNAi [Vienna] was also subjected to immunoprecipitation to confirm the specificity of the anti-dMfn antiserum provided by Dr. Whitworth. ❋ Angela C. Poole Et Al. (2010)
Co-immunoprecipitation of BIN1 and Cav1. 2 (Figure 4B) further indicates that they are present in the same protein complex. ❋ Ting-Ting Hong Et Al. (2010)
Applying GREAT to data sets from chromatin immunoprecipitation coupled with massively parallel sequencing (ChIP-seq) of multiple transcription-associated factors, including SRF, NRSF, GABP, Stat3 and p300 in different developmental contexts, we recover many functions of these factors that are missed by existing gene-based tools, and we generate testable hypotheses. ❋ Cory Y McLean (2010)
Chromatin immunoprecipitation (ChIP) assay confirmed the recruitment of transcription factors phospho ATF-2, Sp1, Sp3, PPARγ, chromatin modifier histone deacetylase 1 (HDAC1) and the acetylated form of histone H3 but not cRel, C/EBP-α and phospho c-Jun during resistin gene transcription. ❋ Anil K. Singh Et Al. (2010)
A powerful technique has recently emerged that couples chromatin immunoprecipitation (ChIP) with next-generation sequencing, (ChIPSeq). ❋ Zhaohui Qin (2010)
This panel also includes co-immunoprecipitation between overexpressed BIN1-V5 (IP) and Cav1. 2 (IB) in HeLa cells. ❋ Ting-Ting Hong Et Al. (2010)
To control for the possible nonspecific interaction of dMfn with our anti-Parkin antiserum, we also subjected a protein extract from park25 null mutants to immunoprecipitation with an anti-Parkin antiserum and then used this immunoprecipitate in a western blot analysis with our anti-dMfn antiserum. ❋ Angela C. Poole Et Al. (2010)
Studies involve freshly acquired human and mouse adult cardiomyocytes using complementary immunocytochemistry, electron microscopy with dual immunogold labeling, and co-immunoprecipitation. ❋ Ting-Ting Hong Et Al. (2010)
DNA sequencer and chromatin immunoprecipitation techniques is desirable. ❋ Unknown (2010)
Additionally, a physical interaction between PPARγ and Sp1 or Sp3 was evident from co-immunoprecipitation experiments. ❋ Anil K. Singh Et Al. (2010)
However, analysis of the known sequences of eukaryotic miRNAs and co-immunoprecipitation experiments indicate that there is a clear bias for U or A at the 5′ position ❋ Filipp Frank (2010)
AKAP5 also associates with PSD-95 and GluR2/3 by immunoprecipitation but the interaction between GluR2/3 and PSD-95 is not disrupted in the AKAP5 KO. ❋ Michael Weisenhaus Et Al. (2010)