Sections were microwaved in a pressure cooker for 15 min before being immunostained on a DAKO autostainer using Vectastain® ABC kits (Vector Labs) according to the manufacturer's protocol. ❋ Malgorzata Romanowska Et Al. (2010)
RGC5 cells were transfected for 20 h to express optineurin-GFP (OPTN-GFP, in green) and immunostained with anti-Rab8, anti-MyoVI, or anti-TfR. Cy3-labled secondary antibody was used and the Rab8, MyoVI and TfR.staining appeared in red (Cy3). ❋ Hongyu Ying Et Al. (2010)
Right panels: B16-GFP and selected B16-GFP melanoma cells grown with high density C2C12 or 10T1/2 fibroblasts and immunostained for MiTF (red). ❋ Ara Parlakian Et Al. (2010)
Multi-photon imaging of the immunostained sections was performed using a Ti: sapphire laser ❋ Nyresa C. Alves Et Al. (2010)
RGC5 cells were transfected for 20 h, fixed and immunostained. ❋ Hongyu Ying Et Al. (2010)
Frozen sections were immunostained for laminin to visualize the basal lamina of the fibers. ❋ Ara Parlakian Et Al. (2010)
Five commercially available antibodies were tested in tissue array sections immunostained utilizing the avidin-biotin (AB) technique. ❋ Unknown (2010)
Samples were fixed in 4%PFA and immunostained with anti-Oct4 and Alexa-488 conjugated donkey anti-mouse IgG to determine germ cells. ❋ Hitomi Suzuki Et Al. (2010)
Human RPE cells were immunostained with anti-C-terminal (Anti-C-term, in green) or anti-INT (Anti-INT, in green) optineurin along with anti-GM130 (in red). ❋ Hongyu Ying Et Al. (2010)
Fifty µm sections were immunostained as described above. ❋ Chihiro Kawaguchi Et Al. (2010)
(B) Collected HSPCs were immunostained using anti human CD34 antibody and analyzed by confocal microscopy. ❋ Sangeetha V. M Et Al. (2010)
GAD65-GFP-positive cells immunostained with antibodies to Ki67, Mash1, GFAP or Olig2. ❋ Beata Jablonska (2010)
Spinal cord sections were stained with H&E to assess inflammation and immunostained for myelin-binding protein (MBP) to evaluate myelin depletion. ❋ Francesca Fallarino (2010)
Then the images were taken using confocal laser microscopy (Zeiss). mRFP intensity in the immunostained embryo did not show significant difference from the unfixed embryo (data not shown). ❋ Hitomi Suzuki Et Al. (2010)
RGC5 cells were double immunostained with anti-C-terminal optineurin (in green) and anti-GM130 (Golgi marker, in red). ❋ Hongyu Ying Et Al. (2010)
(A) The expanded HSPCs were double immunostained with CD34 vs. lineage antibodies like CD33 ❋ Sangeetha V. M Et Al. (2010)
