In the same year, the Gellert lab, which had developed a system to study VDJ recombination in a test tube with purified proteins, discovers a striking similarity between the RAG-mediated reaction and that of known transposases and integrases: both proceed through a characteristic intermediate in which the DNA takes an unusual hairpin-like shape 4. ❋ Unknown (2005)
DNA synapsis and cleavage assays with wild-type and mutant integrases. ❋ Shaohui Liu Et Al. (2010)
DNA cleavage occurs at a 2 bp crossover sequence site has been demonstrated in both phiC31 and Bxb1 integrases ❋ Shaohui Liu Et Al. (2010)
Secondary structure estimation from CD spectra of wild-type and mutant phiC31 integrases. ❋ Shaohui Liu Et Al. (2010)
The complexes observed are integrases (Int) bound to attB or synaptic complexes (SC). ❋ Shaohui Liu Et Al. (2010)
Stoll SM, Sclimenti CR, Lin J, Chen-Tsai Y, et al. (2003) Phage integrases for the construction and manipulation of transgenic mammals. ❋ Shaohui Liu Et Al. (2010)
Bischof J, Maeda RK, Hediger M, Karch F, Basler K (2007) An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases. ❋ Shaohui Liu Et Al. (2010)
Calos MP (2004) Phage integrases: biology and applications. ❋ Shaohui Liu Et Al. (2010)
Assay of DNA binding activity of mutant phi C31 integrases. ❋ Shaohui Liu Et Al. (2010)
Recombination assay of the purified wild-type and mutant phiC31 integrases ❋ Shaohui Liu Et Al. (2010)
Hochhut B, Wilde C, Balling G, Middendorf B, Dobrindt U, et al. (2006) Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536. ❋ Zhemin Zhou Et Al. (2010)
Zambrano N, Mogavero AR, Ammendola R, Cimino F, et al. (1991) A rat brain mRNA encoding a transcriptional activator homologous to the DNA binding domain of retroviral integrases. ❋ Unknown (2009)
Lentiviral integrases will give this vector some tricks that a simple retrovirus, MLV, wont have. ❋ Unknown (2009)
We identified a full spectrum of genetic properties related to its genome plasticity such as repeated sequences, insertion sequences, phage-like proteins, integrases, recombinases and genomic islands. ❋ Unknown (2009)
EIB202 contains 46 genes which are shown to be phage-like products, integrases or recombinases. ❋ Unknown (2009)
Fifty percent (56/112) of the isolates tested were also confirmed as positive for class 1 integrases by PCR. ❋ David W. Verner-Jeffreys Et Al. (2009)
Labelled attB was incubated with wild-type or mutant integrases (Panel A: R18A, I141A; ❋ Shaohui Liu Et Al. (2010)
