Intein

The intein, which is a section of a protein, blocks the part of the enzyme that allows the enzyme to do its work, called the active site. ❋ Jonathan Fahey (2010)

Figure 2: Semisynthesis of Mxe GyrA intein constructs 1-12. ❋ Silvia Frutos (2010)

This doesn't actually disrupt the host's protein, though-amazingly, the intein protein sequence can cut itself out of the surrounding host protein and link its two ends together as if the intein were never there. ❋ John Timmer (2010)

The excised intein protein then helps ensure that the DNA sequence that made it gets inherited and spreads. ❋ John Timmer (2010)

After the plant is harvested, though, the intein can be removed simply by adding heat, exposing the active site and allowing the conversion to biofuels to start. ❋ Jonathan Fahey (2010)

Mycobacterium xenopi DNA gyrase A (Mxe GyrA) intein using protein semisynthesis. ❋ Silvia Frutos (2010)

Kinetic analysis of these molecules indicates that the high fidelity of this protein-splicing reaction results from the penultimate step in the process (intein-succinimide formation) being rate-limiting. ❋ Silvia Frutos (2010)

The new method for developing ubiquitylated proteins, which Zhuang and his team developed, combines the power of intein chemistry and disulfide crosslinking to bond ubiquitin to another essential protein called proliferating cell nuclear antigen. ❋ Unknown (2010)

The self-cleavable intein system (New England Biolabs) was used for overproduction and purification of αCTD and Spx. αCTD (residues 225-314) was overproduced in ❋ Unknown (2010)

Protein splicing is a post-translational modification in which an intein domain excises itself out of a host protein. ❋ Silvia Frutos (2010)

So the company modifies the enzyme slightly, by inserting something called an intein into the enzyme. ❋ Jonathan Fahey (2010)

Native chemical ligation and intein-based methods allow production of circular derivatives of larger proteins, resulting in improved stability and refolding properties. ❋ J. M. Antos (2009)

In this process, a protein sequence (intein) is autocatalytically split off from a fusion protein generated in a cell culture. ❋ Unknown (2009)

Moreover, introduction of the MESNA / diMESNA redox couple in the cleavage buffer allowed simultaneous on-column refolding of Ribonuclease A and intein-mediated cleavage to yield Ribonuclease A with a C-terminal MESNA-thioester. ❋ Unknown (2008)

Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester. ❋ Unknown (2008)

(a) Schematic illustrating conserved regions within the intein family. ❋ Silvia Frutos (2010)

a series of RP-HPLC chromatograms of the reaction mixtures at different time points in the absence of intein. ❋ Silvia Frutos (2010)