Keratocytes

Immunohistochemistry using antibodies specific for UBIAD1 protein in keratocytes revealed that both wild type and N102S protein were localized sub-cellularly to mitochondria. ❋ Michael L. Nickerson Et Al. (2010)

Cell lines were established from patients including keratocytes obtained after corneal transplant surgery and lymphoblastoid cell lines from Epstein-Barr virus immortalized peripheral blood mononuclear cells. ❋ Michael L. Nickerson Et Al. (2010)

To determine the subcellular localization of UBIAD1, keratocytes were further incubated one hour with either 5 µg/ml mouse ❋ Michael L. Nickerson Et Al. (2010)

Chicken anti-UBIAD1 was diluted to 5 µg/ml in FBS blocking solution containing 0.2% Triton X-100, and incubated with keratocytes for one hour at room temperature. ❋ Michael L. Nickerson Et Al. (2010)

For immunofluorescence labeling experiments, the keratocytes were rinsed three times with DPBS before fixing with 2% formaldehyde for 10 minutes at room temperature. ❋ Michael L. Nickerson Et Al. (2010)

Schnyder corneal dystrophy and normal human keratocytes were cultured at 37°C in Fibroblast Medium (catalogue no. 2301) also obtained from ScienCell Research Lab. ❋ Michael L. Nickerson Et Al. (2010)

Normal human keratocytes were purchased from ScienCell Research Lab (Carlsbad, CA). ❋ Michael L. Nickerson Et Al. (2010)

Co-localization of UBIAD1 and OXPHOS complex I mitochondrial marker in keratocytes derived from the Family KK proband (N102S mutation, panels A-C) and a healthy donor (D-F). ❋ Michael L. Nickerson Et Al. (2010)

Co-localization within cultured normal human keratocytes of UBIAD1 protein and protein disulfide isomerase, an enzyme in endoplasmic reticulum, is shown in panels A-C. ❋ Michael L. Nickerson Et Al. (2010)

They appeared to take on the properties of standard corneal cells called keratocytes. ❋ Unknown (2009)

This primary labeling of UBIAD1 protein was then visualized by incubating keratocytes with 5 µg/ml Alexa 594 (red) streptavidin diluted in DPBS (catalog no. ❋ Michael L. Nickerson Et Al. (2010)

Localization within cultured normal human keratocytes of UBIAD1 and protein disulfide isomerase, an enzyme marker for the endoplasmic reticulum, is shown ( ❋ Michael L. Nickerson Et Al. (2010)