Ligases

APG had made good progress with a new process of sequencing that used a technique for gluing strands of DNA together rather than synthesizing new strands, using enzymes called ligases, that bond the fragments together. ❋ Kevin Davies (2010)

Kotorashvilli A, Russo S, Mulugeta S, Guttentag S, Beers M. Anterograde transport of surfactant protein C proprotein to distal processing compartments requires PPDY mediated association with Nedd4-2 ubiquitin ligases. ❋ Unknown (2010)

Speaking of undersampling, it would have been good to look at the breakdown of common substrates of DD-peptidyl ligases and beta lactamases, such as m-[[phenylacetyl-glycyl]oxy]benzoic acid and see what the muations did to these activities. ❋ Unknown (2007)

Of course a ligase is also a type of enzyme, so for Berlinski to make a claim about ligases while excluding enzymes is nonsensical. ❋ Unknown (2006)

Animal HECT ubiquitin ligases: evolution and functional implications ❋ Unknown (2010)

Restriction enzymes and ligases therefore allow the specific cutting and reassembling of portions of DNA. ❋ Unknown (2010)

Fragments of DNA generated in this way can be joined using other enzymes called ligases. ❋ Unknown (2010)

Proteins of the PIAS family can act as SUMO-3 ligases, significantly enhancing the level of the substrate sumoylation ❋ Mara Marongiu Et Al. (2010)

The complex evolutionary history of HECT ubiquitin ligases in animals has been deciphered. ❋ Unknown (2010)

HECT ubiquitin ligases (HECT E3s) are key components of the eukaryotic ubiquitin-proteasome system and are involved in the genesis of several human diseases. ❋ Unknown (2010)

In outline, sumoylation involves the reversible covalent attachment of a SUMO protein moiety (about 11 kDa) to lysine residues in proteins through a three-step process, engaging the E1-activating molecules SAE1/SAE2, the E2-conjugating enzyme UBC9, and E3 ligases such as PIAS in mammalian cells that confer specificity ❋ Mara Marongiu Et Al. (2010)