In addition, Pro74, Ala105 and Gly113 were all amino acids that have been described as important for the multimerization and function of LasR ❋ Thomas Bjarnsholt Et Al. (2010)
Although the association of HSF1 with protein chaperones is one mechanism for its regulation, HSF1 is also thought to engage in intramolecular coiled-coil interactions that maintain the monomeric state and point mutations in these coiled-coil domains (leucine zippers) cause constitutive multimerization when expressed in mammalian cells ❋ Daniel W. Neef Et Al. (2010)
HSF1A stimulates human HSF1 multimerization in yeast and activates human HSF1-dependent yeast cell growth. ❋ Daniel W. Neef Et Al. (2010)
Utilizing this screen, we have identified HSF1A, a molecule capable of promoting human HSF1-dependent yeast cell growth, HSF1 multimerization in yeast, as well as HSF1-dependent protein chaperone expression in mammalian cell culture and in fruit flies. ❋ Daniel W. Neef Et Al. (2010)
Because the inability of HSF1 to complement the viability defect of yhsfΔ cells is due to defective HSF1 multimerization in yeast, cross-linking experiments were conducted to ascertain whether HSF1A promotes increased HSF1 multimerization in yeast. ❋ Daniel W. Neef Et Al. (2010)
(E) Yeast strain DNY75 was grown in the presence of DMSO or 20 µM HSF1A for 18 h, and HSF1 multimerization was evaluated by EGS cross-linking, SDS-PAGE, and immunoblotting. ❋ Daniel W. Neef Et Al. (2010)
Previous studies demonstrated that in response to proteotoxic stress mammalian HSF1 is activated in a multistep process that involves multimerization, nuclear accumulation, hyperphosphorylation, and DNA binding to promoter HSEs to activate protein chaperone gene expression ❋ Daniel W. Neef Et Al. (2010)
HSF1 multimerization state was assessed using the amine-specific cross-linker ethylene glycol bis-succinimidyl succinate (EGS) (Pierce). ❋ Daniel W. Neef Et Al. (2010)
In response to proteotoxic stress or pharmacological inhibitors of Hsp90, this complex dissociates, resulting in the multimerization of HSF1 ❋ Daniel W. Neef Et Al. (2010)
However, further research is needed to identify if mutation of these conserved residues located within a cysteine-rich motif and the leucine-and valine-rich region in the C-terminal domain of phiC31 integrase will affect multimerization characteristic of these proteins. ❋ Shaohui Liu Et Al. (2010)
Wolgamot G, Miller AD (1999) Replication of Mus dunni endogenous retrovirus depends on promoter activation followed by enhancer multimerization. ❋ Unknown (2009)
These antibodies, termed "trimerbodies", use the N-terminal association subdomain of collagen XVIII NC1, responsible for the non-covalent trimerization of collagen alpha chains, to drive multimerization ❋ Unknown (2009)
A mutant HIV-1 Gag that exhibits reduced RNA binding also failed to reconstitute BiFC with wild-type A3G, indicating a requirement for both HIV-1 Gag and A3G to bind to RNA for their multimerization. ❋ Yeshitila Friew (2009)
The results obtained with catalytic domain 1 and 2 (CD1 and CD2) mutants indicate that A3G-A3G and A3G-Gag multimerization is dependent on an intact CD1 domain, which is required for RNA binding. ❋ Yeshitila Friew (2009)