Myotubularin

We provide examples of the dynamic oligomerization of GFP-tagged myotubularin (MTM1) phosphoinositides phosphatase induced by osmotic stress, and of the ultrastructure of membrane tubules dependent on amphiphysin 2 (BIN1) expression. ❋ Unknown (2010)

They do not correspond to membrane tubules, as hypothesized from the known function of myotubularin, but represent most likely oligomers, that were previously described Correlative light and electron microscopy of the needle-like structures. ❋ Coralie Spiegelhalter Et Al. (2010)

(A) COS-1 cells were transfected with GFP or YFP-tagged wild-type myotubularin (MTM1) or close homologous proteins MTMR2 and MTMR4, either untreated or switched to a hypo-osmotic medium for ❋ Coralie Spiegelhalter Et Al. (2010)

We were able to record at precise time points GFP-tagged myotubularin oligomerization in osmotically challenged living cells. ❋ Coralie Spiegelhalter Et Al. (2010)

To exemplify the accessibility of the method, we characterized the oligomerization of GFP-tagged myotubularin induced by hypo-osmotic treatment at the ultrastructural level. ❋ Coralie Spiegelhalter Et Al. (2010)

Schaletzky J, Dove SK, Short B, Lorenzo O, Clague MJ, et al. (2003) Phosphatidylinositol-5-phosphate activation and conserved substrate specificity of the myotubularin phosphatidylinositol 3-phosphatases. ❋ Coralie Spiegelhalter Et Al. (2010)

Some PTP members of the PTEN and myotubularin families have evolved to dephosphorylate phosphatidylinositol phosphates specifically ❋ Unknown (2009)

The second PTP domain (D2) of many classical receptor-like PTPs and the PTP domain of dual-specificity pseudophosphatases of the myotubularin family lack critical residues essential for their catalytic activity ❋ Unknown (2009)

This nucleation is enhanced by the GFP tag, as untagged proteins usually retain normal localization, and is dependent on the myotubularin sequence as the localization of close homologues is not similarly affected ( ❋ Coralie Spiegelhalter Et Al. (2010)