Nutrose

Spread several sets of surface plates upon nutrose agar, each from one drop of the original milk sample. ❋ Unknown (N/A)

If the growth is impure, replate on nutrose agar, pick off colonies and subcultivate again. ❋ Unknown (N/A)

Heat over a bunsen flame, shaking constantly until the fluid boils, and the nutrose is dissolved. ❋ Unknown (N/A)

Add the nutrose ascitic solution to the fluid agar. ❋ Unknown (N/A)

Prepare surface plate cultivations upon nutrose agar from each tube that shows growth either macroscopically or microscopically, and incubate for twenty-four hours aerobically at 37° C. 13. ❋ Unknown (N/A)

Use 0.1 c.c. of the mixed liquor to inseminate each of a series of three nutrose surface plates. ❋ Unknown (N/A)

Liquefy three tubes of nutrient agar -- nutrose agar or the like. ❋ Unknown (N/A)

Mix the agar emulsion and the peptone-nutrose solution in a "tared" flask of 2-litre capacity and add a further 100 c.c. serum water. ❋ Unknown (N/A)

If apparently M. melitensis, inoculate growth from a nutrose agar culture after three days incubation intracranially into the guinea-pig. ❋ Unknown (N/A)

Pick off suspicious colonies, examine them microscopically and subcultivate upon nutrose agar in tubes; upon glucose agar and in litmus milk. ❋ Unknown (N/A)

Media, preparation of nutrient bouillon, 163 gelatine, 164 nutrose agar (Eyre), 172 oleic acid agar (Fleming), 201 ❋ Unknown (N/A)

Incubator running at 37° C. Surface plates of nutrose agar (see page 232). ❋ Unknown (N/A)