That light gets funneled into a crystal of a material called neodymium-doped yttrium orthovanadate, which is common to lab lasers. ❋ Unknown (2010)
The yttrium orthovanadate (YVO4) is a positive uniaxial crystal grown with Czochralski method. ❋ Unknown (2010)
To assess ERK phosphorylation, animals were exposed to a light pulse (20 or 100lx) or no pulse (0lx) at CT15, and then perfused with 4% paraformaldehyde in PBS including 1 mM sodium orthovanadate and 0.2 mM phenylmethyl sulfonyl fluoride to inhibit phosphatase activity, at an appropriate time after light stimulation (7.5 min for the 20lx stimulus and 15 min for the 100lx stimulus). ❋ Chihiro Kawaguchi Et Al. (2010)
Recombinant human ERK1-GST (Upstate Biotech) was nitrated with 100 µM SIN-1 in buffer containing 20 mM MOPS (pH 7.2), 25 mM β-glycerol phosphate, 5 mM EGTA, 1 mM sodium orthovanadate, with 15 mM MgCl ❋ Alfredo Csibi Et Al. (2010)
Inside a green laser pointer, infrared light from a semiconductor diode laser pumps infrared light at a wavelength of 808 nm into a transparent crystal of yttrium orthovanadate doped with neodymium atoms (Nd: YVO4), causing the crystal to lase even deeper in the infrared, at 1064 nm. ❋ Unknown (2010)
DTT, phosphatase inhibitors (0.1 µM okadaic acid and 1 mM sodium orthovanadate) and protease inhibitors (1 mM PMSF and 10 µg/ml leupeptin) using a motorized teflon-glass homogenizer. ❋ Pramod K. Dash Et Al. (2010)
Protein samples from culture wells were collected as described from microwell plates, and lysed in WB-buffer (25 mM Hepes pH 7.5, 100 mM NaCl, 5 mM EDTA pH 8.3, 0.5% Triton X-100, 20 mM β-glycerophosphate, 100 µM orthovanadate, 0.5 mM PMSF and 1 mM DTT). ❋ Ville Härmä Et Al. (2010)
Their results are supported by the fact that the well described PTP inhibitor sodium orthovanadate abrogated 60\% of this activity. ❋ Unknown (2009)
Cells were washed once in PBS and lysed in RIPA buffer (150 mM NaCl, 1\% Triton X100, 0. 5\% sodium deoxycholate, 0. 1\% SDS in 50 mM Tris-HCl pH 7.5) supplemented with 10 mM ß-glycerophosphate, 10 mM sodium pyrophosphate, 10 mM sodium fluoride, 1 mM sodium orthovanadate, 3 mM benzamide, 5 µM cantharidine and complete protease inhibitor cocktail ❋ Unknown (2009)
When indicated, 10 mM of the PTP inhibitor sodium orthovanadate (Fisher Scientific, Ottawa, ON) was added to the buffer ❋ Unknown (2009)
Cells were washed three times with PBS containing 1 mM sodium orthovanadate plus 5 mM EDTA and scraped into lysis buffer containing 20 mM Tris-HCl (pH 8.0), 1\% Triton X-100, 10\% glycerol, 100 mM NaCl, 2 mM EDTA, 1 µM PMSF, 20 µM leupeptin, and 0.15 U / ml aprotinin. ❋ Unknown (2008)
Inhibition of tyrosine phosphatase activity by phenylarsine oxide or sodium orthovanadate shifted ventricular hyperpolarization-activated current (If, generated by HCN channels) activation from nonphysiological voltages into physiological voltages associated with accelerated activation kinetics. ❋ J. Huang (2008)
Triton X-100, 0. 5% sodium deoxycholate), and protease and phosphatase inhibitors (1 µg/ml leupeptin, 3 µg/ml aprotinin, 40 µg/ml soybean trypsin inhibitor, 0.5 mM 4 - (2-aminoethyl) bezenesulfonyl fluoride, 0.1 µM microcystin-LR, 0.2 mM NaF, 0.2 mM orthovanadate). ❋ Michael Weisenhaus Et Al. (2010)
