The bacteria contain a palette of mutations that render them particularly susceptible to plasmid replication, and have been chemically permeabilized for transfer of the DNA across the membrane and cell wall in a procedure known as transformation. ❋ Unknown (2005)
CCE cells were fixed with 4% paraformaldehyde for 10 min, washed, and permeabilized with methanol. ❋ Tzu-Ching Chang Et Al. (2010)
This solution was used like a primary antibody in immunofluorescence staining, and was incubated with PFA-fixed saponin permeabilized cells at 37°C for 1 hour. ❋ Yang O. Zhao Et Al. (2010)
Cells were fixed, permeabilized and stained with goat anti-mouse F (ab) 2 Alexa 488. ❋ Ulrike Naumann Et Al. (2010)
Caremani M, Piperio C, Brandt P, Lombardi V (2007) Stiffness and fraction of Myosin motors responsible for active force in permeabilized muscle fibers from rabbit psoas. ❋ Shenping Wu Et Al. (2010)
For confocal microscopy of the cryosections, nuclei of fixed and permeabilized cryosections were stained with DAPI. ❋ Ulrike Naumann Et Al. (2010)
We show that the αK helix could be also used in the form of a synthetic biotinylated peptide to target a labelled streptavidin complex to Golgi membranes in fixed, permeabilized cells. ❋ Yang O. Zhao Et Al. (2010)
Although the membrane-disrupting activity of NP4P was slight or undetectable, ASABF-alpha permeabilized S. aureus membranes with enhanced efficacy in the presence of NP4P. ❋ Unknown (2010)
The cells were fixed and permeabilized with Cytofix/Cytoperm ™ buffer (BD BioScience) for 20 minutes at 4°C and incubated with allophycocyanin anti-IFN-γ or corresponding isotype controls (eBioscience) for 20 minutes on ice. ❋ Xiaoling Gao Et Al. (2010)
After washing the cells again with PBS, we permeabilized the cells with 0. 1% Tween 20 in PBS for 1 hour, blocked the cells with PBS containing 2% normal serum for 1 hour, and incubated the cells with primary antibodies for 2 hours at room temperature. ❋ Yonghui Zhang Et Al. (2010)
Cells were fixed in 4% paraformaldehyde, permeabilized with chilled 50% methanol (as per requirement) and blocked with PBS containing 1% BSA for 1 hour. ❋ Sangeetha V. M Et Al. (2010)
For this the cells were fixed and permeabilized using BD fixperm kit (BD pharmingen - San Jose, California) and the staining was done as per manufacturer's instructions. ❋ Sangeetha V. M Et Al. (2010)
LC (2002) A model of cross-bridge attachment to actin in the A*M*ATP state based on x-ray diffraction from permeabilized rabbit psoas muscle. ❋ Shenping Wu Et Al. (2010)
Nuclear transport is a biological topic in which SPT measurements have given several quantitative results in both permeabilized ❋ Francesco Cardarelli Et Al. (2010)
The stained cells were fixed and permeabilized and counter stained with anti bcl-2 antibody and analysed on FACS. ❋ Sangeetha V. M Et Al. (2010)
Cells fixed at room temperature for 1 h in Histochoice were unmasked in Citrate Buffer solution for 20 sec in a microwave, permeabilized with 20 mM Tris-HCl, pH 7.6, 137 mM NaCl, and 0. 1% Tween 20 containing 5% skim milk for 30 min at room temperature (according to Hattori et al., ref. 6) and incubated with primary antibodies overnight at 4°C. ❋ Mara Marongiu Et Al. (2010)
At 3 hpi, cells were harvested, fixed in 0. 5% PFA, permeabilized in 0. 1% ❋ Christopher R. Cotter Et Al. (2010)
The cells were permeabilized with 0.3% Triton X-100 in PBS for 10 min and incubated with 5% bovine serum albumin (BSA) in PBS for 30 min to block nonspecific antibody binding. ❋ Shigeyuki Kanazawa Et Al. (2010)
