Phosphotransfer

PknJ-KD-K43A showed no phosphotransfer (data not shown). ❋ Gunjan Arora Et Al. (2010)

Due to very low in vitro activity of PknJ-FL, marginal phosphotransfer by observed on MyBP (lane2). ❋ Gunjan Arora Et Al. (2010)

In vitro autophosphorylation of full length kinase (PknJ-FL) (2 µg) and phosphotransfer on 5 µg Myelin basic protein (MyBP). ❋ Gunjan Arora Et Al. (2010)

In vitro autophosphorylation of PknJ-KD (1 µg) and phosphotransfer on 5 µg Myelin basic protein (MyBP). ❋ Gunjan Arora Et Al. (2010)

It may be attributed to the inefficient phosphotransfer on the immobilized - substrates by GST-PknJ-FL. ❋ Gunjan Arora Et Al. (2010)

Time-dependent phosphorylation of mtPykA by PknJ-KD was performed to investigate efficiency of phosphotransfer. ❋ Gunjan Arora Et Al. (2010)

Typically, a chemical or other stimulus is detected by the periplasmic sensor domain of a transmembrane histidine kinase sensor, which in turn relays a signal through a phosphotransfer cascade to the cognate cytoplasmic response regulator. ❋ J. Cheung (2008)