Phototoxicity

Furthermore, there are other limitations in fluorescence microscopy, such as phototoxicity and imaging setup complexity. ❋ Unknown (2009)

Moreover unlike small organic fluorescent molecules, GFP evolved over the eons to have relatively low phototoxicity. ❋ William Harryman (2009)

The rise of two-photon imaging has allowed the study of the live brain tissues in situ over periods of days to months with little or none of the phototoxicity effects that limited previous methods. ❋ William Harryman (2009)

Second, confocal detection is inherently inefficient, often requiring more illumination of the live specimen than it can endure before bleaching or phototoxicity occurs. ❋ William Harryman (2009)

Sunlight falling on the dispersed oil may make the problem worse through a phenomenon known as phototoxicity. ❋ Unknown (2010)

Ultraviolet treatment of the lens is, however, not possible in a clinical setting because of retinal phototoxicity Instead, we chose to explore a multiphoton approach where ultraviolet-like effects are obtained in confined space using infra-red light that is less harmful to the retina ❋ Line Kessel Et Al. (2010)

The topics of interest cover the use of light for therapeutic and diagnostic purposes - from fundamental principles to development and application - and extend to the interaction of light with biological material at the molecular, membrane, cell and organ levels (including phototoxicity and photosensitizer molecules and particles), as well as to novel optical imaging techniques (including functional imaging for applications in life sciences). ❋ Unknown (2009)

Furthermore, the limited excitation region reduces phototoxicity because photodamage is largely confined to the focal volume.

Also, some dermatologists believe that home phototherapy is inferior to hospital treatment and carries more risks, such as inaccurate dosimetry, phototoxicity, and unsupervised continuation of irradiation. ❋ Unknown (2009)

This technique, however, has a number of drawbacks such as the limited number of available fluorescent channels in microscopes, overlapping excitation and emission spectra of the stains, and phototoxicity. ❋ Unknown (2009)