Preimmune

The control samples were treated with the preimmune serum. ❋ Jie Chen Et Al. (2010)

Cryosections and tissues immunostained with preimmune serum were used as negative controls (Aiii-Eiii). doi: 10.1371/journal. pone.0010133.g003 ❋ Jie Chen Et Al. (2010)

Fibroblasts stably infected with a retroviral construct expressing hemagglutinin (HA) - tagged MEOX2 were harvested 15 days postinfection and subjected to chromatin immunoprecipitation with HA, PolII, or preimmune IgG antibodies. ❋ Unknown (2009)

Immunocytochemical stainings were performed on cytocentrifuged rat (RBL-1) and human (HMC-1 and LAD2) MCs using the two rabbit antisera against AZIN2 and the corresponding preimmune sera. ❋ Kristiina Kanerva Et Al. (2009)

To further validate the antisera, COS-7 cells were grown on coverslips, transfected with FLAG-tagged AZIN2, AZIN1, or ODC for 24 h, and stained with mouse anti-FLAG antibody together with either antiserum 2 or 3, and the corresponding preimmune sera. ❋ Kristiina Kanerva Et Al. (2009)

AZIN2-FLAG-transfected cells were also stained with preimmune sera of the corresponding rabbits. ❋ Kristiina Kanerva Et Al. (2009)

Whole sera from the immunized rabbits were used for immunofluorescence stainings and negative controls were prepared by substituting the primary antibodies with preimmune rabbit sera. ❋ Kristiina Kanerva Et Al. (2009)

Human skin tissue sections stained histochemically with either antiserum 3 to AZIN2 (A) or rabbit preimmune serum (B). ❋ Kristiina Kanerva Et Al. (2009)

Whole lysates of mouse organs (brain, liver, kidney, spleen, heart, and lungs), embryonic day 12 embryos, and cultured fibroblasts, clarified by high-speed centrifugation, were preabsorbed on the column with coupled preimmune serum. ❋ Unknown (2008)

All control stainings with the preimmune sera were negative ( ❋ Kristiina Kanerva Et Al. (2009)