Sepharose

Nuclear extracts from unstimulated U937 cells were incubated individually with antibodies against Sp1, Sp3 and PPARγ followed by mopping of the immuno-complex with Protein G sepharose beads. ❋ Anil K. Singh Et Al. (2010)

The sepharose beads were washed 3 times with 800 µl ice-cold RIPA buffer and resuspended in 60 µl 2× sample buffer, followed by elution through boiling. ❋ Anil K. Singh Et Al. (2010)

The supernatant was collected in fresh tubes and pre cleared with Protein G sepharose equilibrated with salmon sperm DNA for 30 minutes at 4°C. ❋ Anil K. Singh Et Al. (2010)

The Protein G sepharose beads were washed with cold ChIP sonication buffer containing no protease inhibitors. ❋ Anil K. Singh Et Al. (2010)

Protein concentration of the dialyzed nuclear extracts was determined by the Bradford method. 30 µg of the dialyzed nuclear extracts were incubated with 2 µg each of anti-Sp1, anti - Sp3 and anti-PPARγ antibodies along with Protein G sepharose beads for 4 hours at 4°C with gentle shaking. ❋ Anil K. Singh Et Al. (2010)

The cell lysates were centrifuged at 14 K rpm, 4°C for 20 min. The supernatant containing recombinant protein was collected and incubated with glutathione sepharose 4B affinity resin (GE Healthcare Bio-Sciences) pre-equilibrated with buffer A. ❋ Gunjan Arora Et Al. (2010)

For the biofilm inhibition assay, rabbit antibodies were purified using protein G sepharose, according to the manufacturer's instructions (Ab Spin Trap, GE Healthcare) and tested for their ability to inhibit biofilm formation. ❋ Cira Daniela Rinaudo Et Al. (2010)

The cell lysate was pre-cleared by adding 100 µl of 50% Protein G sepharose bead slurry per 1 ml of cell lysate. ❋ Anil K. Singh Et Al. (2010)

The immune complex was captured by the addition of 100 µl Protein G sepharose. ❋ Anil K. Singh Et Al. (2010)

The immune complex was precipitated by incubation of the samples with Protein G sepharose equilibrated with salmon sperm DNA at ❋ Anil K. Singh Et Al. (2010)

Fat Loss 4 Idoits pharmacia q sepharose resin! major producers of epoxy resin ❋ Unknown (2009)

Rabbit IgG were purified using protein-G sepharose column (GE Healthcare, USA). ❋ Unknown (2009)

For immunoprecipitation of SR-BI / CLA-1, 5 µl of rabbit anti-SR-BI antibodies (Novus NB400-104) or rabbit non-immune serum were mixed with 25 µl of 400 mg / ml protein A-sepharose (Healthcare Biosciences) in Tris buffer ❋ Unknown (2009)

After several washings with Tris buffer, SR-BI antibodies combined to protein A-sepharose were incubated with 90 µg of Caco-2 / TC7 cell homogenates, for 2 hours at ❋ Unknown (2009)

Antigen pre-treatments were performed by incubating purified antibodies with the antigen immobilized to glutathione sepharose beads for 24 hours followed by the removal of beads using 0.22 µm spin-X cellulose acetate filters. ❋ Unknown (2009)