Sonicate

M. tuberculosis-sonicate antigens increased specific luciferase activity in a dose-dependent manner and to a level greater than that induced by ❋ Malathesha Ganachari Et Al. (2010)

M. tuberculosis-sonicate increased the expression of MMP-1 mRNA in a dose-dependent fashion and to higher levels than in cells stimulated with antigen alone (Kruskal-Wallis Figure 3C). ❋ Malathesha Ganachari Et Al. (2010)

The amount of sonicate added per flask of stimulated cells originated from one flask of RTHDF fibroblasts. ❋ Hans-Christian Ingerslev Et Al. (2010)

M. tuberculosis-sonicate antigens alone, with the exception of cells stimulated with MCP-1 increases MMP-1 expression inTHP-1 monocytic cells stimulated by We measured the relative changes in MMP-1 gene expression by real-time PCR. ❋ Malathesha Ganachari Et Al. (2010)

M. tuberculosis-sonicate antigens and human rMCP-1. ❋ Malathesha Ganachari Et Al. (2010)

The sonicate was then centrifuged for 2 min at 14. 000× g and the clear supernatant was transferred to a new tube. ❋ Hans-Christian Ingerslev Et Al. (2010)

M. tuberculosis-sonicate was used to stimulate the cells for the experiments shown in B, C, and D. ❋ Malathesha Ganachari Et Al. (2010)

M. tuberculosis-sonicate antigen stimulated (+) THP-1 cells that were cultured for 48 hours in serum-free media with or without increasing amounts of human rMCP-1. ❋ Malathesha Ganachari Et Al. (2010)

M. tuberculosis-sonicate antigens, human rMCP-1, or both. ❋ Malathesha Ganachari Et Al. (2010)

Cells were stimulated overnight with or without M. tuberculosis-sonicate antigens and with the indicated amounts of human recombinant MCP-1. ❋ Malathesha Ganachari Et Al. (2010)

M. tuberculosis-sonicate antigens or human rMCP-1 and expression of ❋ Malathesha Ganachari Et Al. (2010)

M. tuberculosis-sonicate antigens to higher levels than in carriers of the other two-locus ❋ Malathesha Ganachari Et Al. (2010)

M. tuberculosis-sonicate antigens plus rMCP-1 was always significantly greater than in that culture media from cells stimulated with antigen alone. ❋ Malathesha Ganachari Et Al. (2010)

M. tuberculosis-sonicate plus the various concentrations of rMCP-1 than in cells stimulated with ❋ Malathesha Ganachari Et Al. (2010)

The sonicate was prepared by an initial trypsination of confluent cell culture flasks followed by sonication for 30 s (amplitude 10 microns) using a MSE Soniprep 150 sonicator (Sanyo). ❋ Hans-Christian Ingerslev Et Al. (2010)

M. tuberculosis-sonicate antigens alone or in the presence of human rMCP-1. ❋ Malathesha Ganachari Et Al. (2010)

In line, Verreck et al. showed that Mφ1 produce IL-23 and IL-1β in response to LPS or mycobacterial sonicate ❋ Diederik Van De Wetering Et Al. (2009)

The fact that IL-8 was not significantly upregulated by the sonicate suggests that there may be a specificity in the spirochete attachment process that is involved in generating IL-8 expression. ❋ Ram Naresh Et Al. (2009)

Using their powerful thoracic muscles carpenter bees sonicate the dry pollen grains out of the flower’s anthers. ❋ Unknown (2008)