Sortase

By contrast, loss of the major ancillary protein AP1 or of the sortase SrtC-2 responsible for AP1 incorporation in the pilus does not affect the ability of bacteria to adhere to surfaces. ❋ Cira Daniela Rinaudo Et Al. (2010)

Taken together, these data indicated that the presence of a polymerized pilus, correctly anchored to the cell wall, a process that involves both ancillary protein AP2, sortase SrtC-1 and Sortase A, is necessary for efficient biofilm formation. ❋ Cira Daniela Rinaudo Et Al. (2010)

BP-2a gene; (D) ΔsrtC-1, strain 515 ΔsrtC-1 with in-frame deletion of the sortase srtC-1 gene; (E) ΔsrtC-2, strain 515 ΔsrtC-2 with in-frame deletion of the sortase srtC-2 gene; (F) ❋ Cira Daniela Rinaudo Et Al. (2010)

ΔsrtC-1/2, strain 515 ΔsrtC-1/2 with in-frame deletion of both sortase genes; (G) ❋ Cira Daniela Rinaudo Et Al. (2010)

Contribution of pilus 2a subunits and sortase A in biofilm formation. ❋ Cira Daniela Rinaudo Et Al. (2010)

However, the biofilm-defective phenotype shown by the mutant strain for the minor ancillary AP2 is consistent with the well demonstrated role of this protein as the anchor protein utilized by sortase A in covalent anchoring of pilus 2a to the cell wall ❋ Cira Daniela Rinaudo Et Al. (2010)

Moreover, we present data showing that the minor ancillary protein of pilus 2a, whose role in pilus anchoring to the cell wall, a process mediated by the constitutive sortase A has been recently demonstrated ❋ Cira Daniela Rinaudo Et Al. (2010)

According to this, also the sortase A mutant strain showed a defective phenotype as well as the KO strain lacking the sortase C specific for AP2 incorporation into pilus. ❋ Cira Daniela Rinaudo Et Al. (2010)

Here we show that circular proteins can be made reversibly with excellent efficiency by means of a sortase-catalyzed cyclization reaction, requiring only minimal modification of ❋ J. M. Antos (2009)

Pilin-specific sortase cleaves the sorting signal of SpaA and SpaB at the threonine residue ❋ Unknown (2008)

The next area where π and θ are positive is the one containing the genes encoding the sortase enzymes. ❋ Alessandro Muzzi Et Al. (2008)

The transient product of this reaction is an acyl enzyme that, depending on the sortase involved, is relieved by a specific nucleophile (4). ❋ Unknown (2008)

[22], as well as the loss of the AP1-specific sortase-C ❋ Cira Daniela Rinaudo Et Al. (2010)

ΔsrtC-1, ΔsrtC-2 and ΔsrtC-1/2 and for sortase A (ΔsrtA ❋ Cira Daniela Rinaudo Et Al. (2010)