The team built the spider by starting with a protein called streptavidin, that conveniently has four symmetrically-placed binding pockets for a chemical called biotin. ❋ Unknown (2010)
To build the 4-nm-diameter molecular robot, the researchers started with a common protein called streptavidin, which has four symmetrically placed binding pockets for a chemical moiety called biotin. ❋ Unknown (2010)
We show that the αK helix could be also used in the form of a synthetic biotinylated peptide to target a labelled streptavidin complex to Golgi membranes in fixed, permeabilized cells. ❋ Yang O. Zhao Et Al. (2010)
Figure 6 shows the staining pattern of Cy3 labelled streptavidin-Irgm1 αK tetrameric complexes in MEFs. ❋ Yang O. Zhao Et Al. (2010)
The Cy3-labelled streptavidin (Sigma, S6402) was first diluted in the blocking buffer to a final concentration of 20 µg/ml (0.33 µM). ❋ Yang O. Zhao Et Al. (2010)
The scientists then coated the micrometer-long, pear-shaped bacteria with a solution containing biotin, a vitamin that readily binds to streptavidin. ❋ Unknown (2006)
As the microbes passed each of a rotor's supporting ridges, their biotin-treated cell membranes clung to the streptavidin coating, causing tugs on the tabs and thereby turning the rotor. ❋ Unknown (2006)
The researchers laid down fetuin within the circular groove and coated the rotor with a protein called streptavidin. ❋ Unknown (2006)
Multiplexes were developed using an excess of streptavidin-phycoerythrin solution which was thoroughly mixed into each multiplex and incubated for 1 hour at room temperature. ❋ Enrico Domenici Et Al. (2010)
Antibody binding was visualized by using biotin-conjugated goat anti-mouse IgG followed by Cy3-conjugated streptavidin (Jackson Immunoresearch), and Alexa488 or Cy3 - conjugated conjugated goat anti-rabbit IgG (Molecular Probes). ❋ Ara Parlakian Et Al. (2010)
(Dako Liquid DAB-substrate - Chromogen system K3468, Carpintera, CA, USA) for streptavidin peroxidase and the fast red Working solution (CHROM) (Dako, Carpintera, CA, USA) for alkaline phosphatase. ❋ Michele Mishto Et Al. (2010)
The spider's 'body' is a common protein called streptavidin. ❋ Unknown (2010)
Due to low amount of IgA in mucosal fluids, a high sensitive sandwich ELISA assay was set; sensitivity was enhanced by milk-based overcoating buffer and by a two-step biotin-streptavidin signal amplification. ❋ PLoS ONE (2010)
D constructs in the presence of ultraviolet light, followed by streptavidin-based purification, stringent washing to remove non-covalent binders, elution and ❋ Michelle L Stewart (2010)
