Figure 4D; supershifting experiments in RSV infected IKKγ ❋ Ping Liu Et Al. (2009)
Note that addition of anti-SP-3 antibody into the reaction also led to formation of the supershifting SP-3 complex. ❋ He-Ge Chen Et Al. (2009)
This supershifting complex did not appear when the normal IgG was used (lane 4, 9 of Fig. 5B). ❋ He-Ge Chen Et Al. (2009)
Lane 5, the same assay as described in lane 1 except that normal IgG was added into the reaction to conduct mock supershifting assay. ❋ He-Ge Chen Et Al. (2009)
Note that addition of anti-ETS-1 antibody into the reaction led to formation of the supershifting ETS-1 complex. ❋ He-Ge Chen Et Al. (2009)
For the supershifting experiments, 30 µg of each nuclear extract was incubated with 1×10 ❋ He-Ge Chen Et Al. (2009)
Lane 5, the same assay as in lane 1 except that anti-ETS-1 antibody was added for supershifting assay. ❋ He-Ge Chen Et Al. (2009)
Note that addition of anti-SP-1 antibody into the reaction led to formation of the supershifting SP-1 complex. ❋ He-Ge Chen Et Al. (2009)
Lane 4, the same assay as described in lane 2 except that normal IgG was added into the reaction to conduct mock supershifting assay. ❋ He-Ge Chen Et Al. (2009)
Lane 4, the same assay as in lane 1 except that normal IgG was used for mock supershifting assay. ❋ He-Ge Chen Et Al. (2009)
Lane 4, the same assay as described in lane 1 except that normal IgG was added into the reaction to conduct mock supershifting assay. ❋ He-Ge Chen Et Al. (2009)
Note that addition of anti-CREB antibody into the reaction led to formation of the supershifting CREB complex. ❋ He-Ge Chen Et Al. (2009)
Note that addition of anti-AP-2α antibody into the reaction led to formation of the supershifting AP-2α complex. ❋ He-Ge Chen Et Al. (2009)
However, addition of normal IgG into the same reactions did not produce the supershifting complex (lane 5, 10 of ❋ He-Ge Chen Et Al. (2009)
Addition of the anti-AP-2α antibody into the binding reactions between labeled AP-2α oligo and the nuclear extracts from the two types of cells induced a supershifting band (lane 5, 10 of ❋ He-Ge Chen Et Al. (2009)
Fig. 6B) but not normal IgG (lane 4 and 10 of Fig. 6B) into the binding reactions between labeled SP-1/SP-3 oligo and the nuclear extracts from the two types of cells induced the supershifting band. ❋ He-Ge Chen Et Al. (2009)
The DNA-ETS-1 complex can be recognized by anti-ETS-1 antibody to form supershifting complex (lane 5 and 10 of ❋ He-Ge Chen Et Al. (2009)
Addition of the anti-CREB antibody into the binding reactions between labeled CREB oligo and the nuclear extracts from both ARPE-19 and FHL124 cells induced obvious supershifting complex (lane 4, 9 of ❋ He-Ge Chen Et Al. (2009)
