Confinement of PPARβ/δ transgene expression to suprabasal epidermal keratinocytes. ❋ Malgorzata Romanowska Et Al. (2010)
B. Nuclear accumulation of PPARβ/δ in suprabasal epidermis in psoriasis skin lesions. ❋ Malgorzata Romanowska Et Al. (2010)
In normal human epidermis, relatively low levels of TGF-β1 are expressed predominantly in the suprabasal, differentiating layers, suggesting it may have a role in maintaining the cessation of growth in the differentiating cells of epidermis During re-epithelialisation, the expression of TGF-β1 is induced by various ECM components. ❋ Salem Adra Et Al. (2010)
To model the suprabasal expression of PPARβ/δ observed in psoriasis in humans, we initially intended to target transgenic PPARβ/δ expression using a "conventional" promoter active in suprabasal epidermis, e.g. the involucrin promoter. ❋ Malgorzata Romanowska Et Al. (2010)
We show that human AD patients have reduced Notch receptor levels on the cell surface of the suprabasal epithelium and they are known to have an increased risk to develop hematological malignancies ❋ Alexis Dumortier Et Al. (2010)
Targeting PPARβ/δ expression to suprabasal skin in mice in vivo ❋ Malgorzata Romanowska Et Al. (2010)
Compared to controls, samples treated with repeated doses of DUVR showed drastic alterations in epidermis (decreased thickness and death of suprabasal keratinocytes) and dermis (decrease in the number of fibroblasts). ❋ Claire Marionnet Et Al. (2010)
Notch receptors are expressed in the suprabasal cell layer of the skin. ❋ Alexis Dumortier Et Al. (2010)
The suprabasal-specific Cldns, Cldn6 (Figure 3E), Cldn11, Cldn12 and Cldn18 (not shown) were seen in the expanded suprabasal region of the Inv-Cldn6-CΔ196 epidermis; each Cldn evaluated maintained a strictly membranous localization and normalized by 1-month to a zone comparable to wild type. ❋ Tammy-Claire Troy Et Al. (2009)
Immunohistochemical analysis revealed aberrant suprabasal Cldn localization with marked down-regulation of Cldn1. ❋ Tammy-Claire Troy Et Al. (2009)
For example, the K14 expression zone was extended into the suprabasal compartment the Inv-Cldn6-CΔ196 epidermis until epidermal thinning was achieved. ❋ Tammy-Claire Troy Et Al. (2009)
It is interesting to note that K6 - and K17-positive suprabasal cells, which are normally not present in the mature epidermis except in situations of abnormal cell proliferation and differentiation ❋ Tammy-Claire Troy Et Al. (2009)
Sporadic Cldn1-positive basal cells were evident in the 2-week old transgenic epidermis; by 1-month of age Cldn1-positive cells occupied both the basal and suprabasal compartments reminiscent of the wild type. ❋ Tammy-Claire Troy Et Al. (2009)
In addition, it has been proposed that aberrant p63 expression may be an early event in the pathogenesis of HNSCC, as extension of p63 expression is observed from normal basal cells into suprabasal levels in mucosal specimens displaying dysplasia ❋ Venkata Sabbisetti Et Al. (2009)
Histological analysis of newborn (A) Inv-Cldn6-CΔ196 transgenic mice as compared to the wild type revealed a moderately thicker epidermis in transgenic samples, with a thicker stratum corneum, a less-compacted granular layer and an overall expanded suprabasal compartment. ❋ Tammy-Claire Troy Et Al. (2009)
To dissect further the mechanisms by which Cldn6 acts during epithelial differentiation, we overexpressed a Cldn6 cytoplasmic tail deletion mutant in the suprabasal compartment of the transgenic mouse epidermis. ❋ Tammy-Claire Troy Et Al. (2009)
Previously we reported that a perturbation of Cldn6 expression levels at its endogenous site of expression - the suprabasal layer of the epidermis - alters epidermal development and the formation of the EPB ❋ Tammy-Claire Troy Et Al. (2009)
Cldn6, a suprabasal-specific Cldn, was observed in an expanded zone early on, and was normalized by ❋ Tammy-Claire Troy Et Al. (2009)
We previously demonstrated that Claudin (Cldn) - containing tight junctions (TJs) are essential in the development of the epidermis and that transgenic mice overexpressing Cldn6 in the suprabasal layers of the epidermis undergo a perturbed terminal differentiation program characterized in part by reduced barrier function. ❋ Tammy-Claire Troy Et Al. (2009)
As already indicated, the precise mechanisms by which expression of low levels of Cldn6-CΔ196 in the suprabasal layers of the epidermis leads to age-related changes appearing to mimic those seen in humans are currently not known, but our observations suggest that changes in Cldn homeostasis leading to changes in the epidermal differentiation program play important roles. ❋ Tammy-Claire Troy Et Al. (2009)
