Transfect

When I started my post doc I tried to transfect bacteria with a construct I got from the tech. ❋ Microbiologist Xx (2008)

You will transfect eukaryotic cells, use immunofluorescent and other techniques to stain mouse tissues and use confocal microscopy. ❋ Unknown (2010)

A key step in bringing this vector to the clinic was to demonstrate that it is both safe and feasible to transfect the vector into human stem cells and for the RNA molecules to persist in the immune cells of the patient. ❋ Unknown (2010)

A plasmid DNA that expresses the green fluorescent protein (GFP) as a marker was delivered into the sub-retinal space to transfect the chick retinal stem/progenitor cells at embryonic day 3 (E3) or E4 with the aid of pulses of electric current. ❋ Unknown (2010)

Hope using the same triple vector to transfect T cells in HIV/AIDS patients who have failed ❋ Unknown (2010)

A related bottleneck is the inability to transfect certain cells types and to load relevant agents such as mRNA, proteins, and small molecules. ❋ Unknown (2009)

DNA, RNA or peptides into cells that are considered difficult or even impossible to transfect. ❋ Unknown (2009)

They state the plasmid DNA was isolated according to the protocols recommended by the different miniprep suppliers before using the plasmid DNA to transfect HEK293T cells. ❋ Unknown (2009)

We constructed a series of AAV helper plasmids containing different Rep78 / 68 start codon in combination with different gene regulatory regulatory sequences. rAAV vectors carrying the human IGF-I gene were prepared with these vectors and the vector preparations used to transfect HT1080 target cells. ❋ Unknown (2009)

"From a technology perspective, their technique to transfect the estrogen receptor is an exciting advance for researching steroid receptors in the brain." ❋ Unknown (2009)

The Maxcyte STX Scalable Transfection System can transfect a few million cells in seconds and up to ten billion cells in less than 30 minutes. ❋ Unknown (2009)

FUCK i got a transfection from [candy]...again, this is [the third] one this [year]!! ❋ Clurebure (2007)