Transfected

Additional features of the EYFP vector presented above are a human cytomegalovirus (CMV) promoter to drive gene expression in transfected human and other mammalian cell lines, and an f1 bacteriophage replication origin for single-stranded DNA production. ❋ Unknown (2005)

But an anti-oncogene, transfected into a cell, cannot be expected to create an “anti-focus.” ❋ Siddhartha Mukherjee (2010)

DNA from these cells was sheared into fragments and transfected into the normal human cell line. ❋ Siddhartha Mukherjee (2010)

Plate after plate of transfected cells piled up in the laboratory. ❋ Siddhartha Mukherjee (2010)

From multiple episomally transfected lines, one showed evidence of K76T substitution in the recombinant, full-length pfcrt locus (data not shown). ❋ Unknown (2007)

Thomas and Capecchi [36] introduced a neomycin resistance gene into an exon of the HPRT gene in ES cells and showed that clones of transfected cells had lost HPRT but gained neoR activity. ❋ Unknown (2007)

All the components were now in place for producing gene-targeted mouse strains: the development of ES cell culture, the demonstration that gene modification in such cells can be transmitted to the germline and registered in the offspring, the observation that homologous recombination occurs with high frequency in the mammalian genome, the application of gene transfer methods to ES cells, and the invention of strategies for enriching transfected cells. ❋ Unknown (2007)

The following year, Capecchi's positive-negative selection strategy for enriching ES cells containing a targeted disruption of any transfected gene was presented in Nature [37] (Fig 2). ❋ Unknown (2007)

The HeLa cells were co-transfected with sub-cellular localization vectors fused to enhanced cyan and yellow (EYFP) fluorescent protein coding sequences (Golgi complex and the nucleus, respectively), as well as a variant of the Discosoma striata marine anemone fluorescent protein, DsRed2FP, targeting the mitochondrial network. ❋ Unknown (2005)

Although transient transfections usually result in the loss of plasmid gene product over a relatively short period of time (several days), stably transfected cell lines continue to produce the guest proteins on a continuous long-term basis (ranging from months to years). ❋ Unknown (2005)

HeLa Cell transfected with three fusion fluorescent proteins. ❋ Unknown (2005)

While frog AID was fully capable of mediating CSR in mouse AID-deficient B cells, they found essentially no CSR when pufferfish AID was transfected remember, frogs have CSR, but pufferfish don't. ❋ Unknown (2006)

In a recent issue of the Journal of Experimental Medicine, Vasco Barreto and colleagues from Michel Nussenzweig's lab reported that the AID gene from zebrafish was capable of activating CSR when transfected into mouse B cells that lacked their own AID gene. ❋ Unknown (2006)

The ganglion cells transfected with the microbial rhodopsin responded to light, depolarised and passed signals on to the visual cortex Bi et al., 2006. ❋ Unknown (2006)

FUCK i got a transfection from [candy]...again, this is [the third] one this [year]!! ❋ Clurebure (2007)