Furthermore, scientists have been routinely transfecting (i.e., inserting) foreign pieces of DNA into all sorts of cells to confer on those recipient cells the abilities to make proteins that they otherwise would not make and to thereby influence, sometimes decisively, their functional properties more broadly. ❋ Unknown (2010)
Colabelling is possible by transfecting cells with different plasmids each containing one fluorescent protein gene and one gene of interest (Figure 3). ❋ Unknown (2005)
It does no one any good to have someone with a degree in physics looking at a patent for a method of transfecting genes into plants. ❋ Peter Zura (2007)
Pps were produced by transfecting 293T cells with four plasmids: an HA expression plasmid, an NA expression plasmid, a Gag-pol-encoding plasmid, and a reporter plasmid. ❋ Yonghui Zhang Et Al. (2010)
Subsequently, Yu et al. [8] obtained iPSCs in humans by transfecting with OCT4, SOX2, NANOG and LIN28. ❋ Simone Pacini Et Al. (2010)
Additional transactivation assays were carried out co-transfecting FOXL2 and increasing amounts of PIAS1 and UBC9 in COS-7 cells, to test whether FOXL2 transcriptional activity, like that of SOX9, is affected by PIAS1 in both SUMO-ligase-dependent and independent manners ❋ Mara Marongiu Et Al. (2010)
To evaluate whether down-regulation of bcl-2 shows opposite effect of bcl-2 overexpression in terms of HIF-1α protein expression, we silenced the endogenous expression of bcl-2 gene transfecting M14 cells with siRNA-targeting bcl-2 mRNA (si-bcl-2) and then exposing them to normoxia or hypoxia for 24 h. ❋ Daniela Trisciuoglio Et Al. (2010)
Esau et al. [9] found that reducing miRNA-143 by transfecting antisense oligonucleotides inhibits adipocyte differentiation in vitro, suggesting that miRNAs may play a role in the regulation of key adipose tissue processes and could therefore represent novel targets for the treatment of obesity. ❋ Unknown (2009)
Obstacles for using synthetic siRNAs include being a nonrenewable resource, the transient nature of silencing, and the difficulty faced in transfecting primary cells and nondividing cell lines such as neurons, lymphocytes, and macrophages. ❋ Unknown (2009)
Pseudotyped lentivirus particles were generated by co-transfecting HEK293T cells with pLOX-CW/Nullbasic-EGFP or pLOX-CWgfp along with pCMVΔR8. 91 ❋ Luke W. Meredith Et Al. (2009)
To test the replication kinetics and coreceptor tropism, viral stock was prepared by transfecting 5 µg HIV-1 clone into 6×10 ❋ Kok Keng Tee Et Al. (2009)
Authenticity of the final product was confirmed by sequencing of the recombinant virus rescued by transfecting the linearized DNA into 293 cells. ❋ Maria A. Croyle Et Al. (2008)
REST-100 ES cells were generated by stably transfecting with the empty pSuper vector, whereas REST / KD-50 ES cells were created by transiently expressing ❋ Yuh-Man Sun Et Al. (2008)
[23] performed excision of multiple integrated lentiviral vectors in hiPSCs by transfecting the cells with a plasmid co-expressing Cre and the puro gene followed by selection of resistant clones. ❋ Cesar Sommer (2010)
[12] transfecting cells with a vector expressing luciferase under the control of 4X Hypoxia Responsive Element (HRE) and another one expressing β-galactosidase under the control of CMV promoter. ❋ Daniela Trisciuoglio Et Al. (2010)
After confirming that FE65 co-precipitated with SET from H4 cell lysates (data not shown), we examined the effects of co-transfecting APP-Gal4 and FE65, with or without Teashirt3 and / or SET, on transcription ( ❋ Unknown (2009)
FUCK i got a transfection from [candy]...again, this is [the third] one this [year]!! ❋ Clurebure (2007)
