The purified plasmid DNA is ready for duty in transfection experiments. ❋ Unknown (2005)
We show here that transient RNAi of endogenous or exogenous gene, which has been achieved after short-term transfection with the gene-targeting 21mer dsRNA (siRNA), 27mer dsRNA or shRNA-generating DNA vector, does not fail for 2-3 days after onset of apoptosis. ❋ Febitha Kandan-Kulangara Et Al. (2010)
A number of methods are in use or under investigation for introducing DNA into cells (a process known as transfection) - using viruses, chemical agents or physical injection. ❋ Unknown (2010)
ELISA, western blot, and molecular biology methods such as transfection and microRNA techniques, high throughput screens, or computer sciences to integrate and analyze genetic, molecular, physicochemical, functional and behavioral data would be viewed as added value. ❋ Unknown (2009)
Shukla AR, Nguyen T, Zheng Y, Zderic SA, DiSanto M, Wein AJ, Chacko S: Over expression of smooth muscle specific caldesmon by transfection and intermittent agonist induced contrac-tion alters cellular morphology and restores differentiated smooth muscle phenotype. ❋ Unknown (2010)
Both of transfection and epidemiological studies suggest that 76th amino acid of PfCRT is critical to CQR parasites. ❋ Unknown (2007)
[AS] – Yes, because the success rate of transfection was very small. ❋ Unknown (2007)
“These nanocarriers developed by our team have a variety of applications in medication and as a gene transfection agent for biological research,” said Dr Yi-Yan Yang, who led the project team comprising Yong Wang, Shujun Gao, Wen-Hui Ye and Ho Sup Yoon. ❋ Unknown (2006)
The co-delivery of small molecular drugs with nucleic acids can improve gene transfection efficiency, reduce side-effects of these drugs, and achieve the synergistic effect of drug and gene therapy for the more effective treatment of cancer. ❋ Unknown (2006)
After failing for along time, trouble shooting and all, I realised that there was no DNA in the tube she'd given me... strange that the transfection didn't work huh? ❋ Microbiologist Xx (2008)
RNA viruses can be replicated in vitro with their own incredibly error-prone replicases and propagated by transfection. ❋ Unknown (2006)
Successful mammalian transfection experiments rely on the use of high quality plasmid or viral DNA vectors that are relatively free of bacterial endotoxins. ❋ Unknown (2005)
The most efficient method of obtaining stable cell lines is to employ a high efficiency technique for the initial transfection. ❋ Unknown (2005)
The choice of transient or stable transfection depends upon the target objectives of the investigation. ❋ Unknown (2005)
In transient, or temporary, gene transfer experiments (often referred to as transient transfection), plasmid or viral DNA introduced into the host organism does not necessarily integrate into the chromosomes, but can be expressed in the cytoplasm for a short period of time. ❋ Unknown (2005)
Mammalian cells used for transfection must be in excellent physiological condition and growing in logarithmic phase during the procedure. ❋ Unknown (2005)
Furthermore, many of these behaviors can be reversed by transfection of the ventral tegmental area (VTA) dopaminergic neurons with WT Clock gene or by treatment with therapeutic doses of lithium (Li+), a commonly prescribed mood stabilizer. ❋ Unknown (2007)
Electroporation applies short, high voltage pulses to a cellular suspension to induce pore formation in the plasma membrane, subsequently allowing the transfection DNA to enter the cell. ❋ Unknown (2005)
Collectively termed lipofection, the lipid-based technology has met with widespread acceptance due to its effectiveness in a large number of popular cell lines, and it is now the method of choice for most transfection experiments. ❋ Unknown (2005)
