After 24 h cultivation, cells were trypsinized, harvested by centrifugation, resuspended in PBS containing 10 µmol/L DHE for 251 min at 37°C and thereafter washed with ice-cold PBS. ❋ Nadine Rohwer Et Al. (2010)
Before chondrogenic induction, trypsinized MSCs and chondrocytes looked similar. ❋ Shizuko Ichinose (2010)
The cells were then trypsinized and induced with 1 µg/ml of Dox for 24 h. ❋ Hongyu Ying Et Al. (2010)
To examine the capability of Y-27632-treated cells to form colonies, Y-27632-treated CCE cells were washed, trypsinized, and reseeded. ❋ Tzu-Ching Chang Et Al. (2010)
VSMCs were trypsinized, seeded at an initial density of 5,000 cells/well in 96-well plates and grown to sub-confluence (2 days). ❋ Jamila Khallou-Laschet Et Al. (2010)
After treatment, cells were trypsinized, and viable cells were counted using a hemocytometer. ❋ Nadine Rohwer Et Al. (2010)
To identify HSF1A-B-interacting proteins, protein bands were excised from the gel, trypsinized to generate peptides, and then subjected to tandem mass spectrometry ❋ Daniel W. Neef Et Al. (2010)
Then the cells were trypsinized, washed and seeded without inhibitors and cytokines for 5 days. ❋ Beatriz Santamaría Et Al. (2009)
At 80% confluence, they were trypsinized and seeded on collagen-coated transwell inserts in 12-well plates ❋ Rebecca M. Green Et Al. (2009)
The cells were washed with 1X PBS and trypsinized. ❋ Sandeep Ravindran Et Al. (2009)
For the FACS analysis, primary fibroblasts were trypsinized immediately before analysis at each time point. ❋ Natalia V. Strunnikova Et Al. (2009)
Twenty-four hours after transfection, cells were trypsinized, plated in 60 mm dishes and selected for 2 weeks using G418. ❋ Unknown (2009)
We printed WGA-biotin-labeled Jurkat and DG-75 suspension cells as well as trypsinized HeLa and HEK293 adherent cells onto replicate chips. ❋ Unknown (2009)
For flow cytometry, cells were trypsinized, collected, and fixed in cold 70\% ethanol at each time point. ❋ Piyali Mukherjee Et Al. (2009)
MSC that reached 80\% confluency were trypsinized and washed three times with PBS, then 1×10 ❋ Unknown (2009)
Myoblasts, upon reaching 80\% confluence, were trypsinized and mounted on microscope slides for homogeneity estimation with IHC staining using the myoblast-specific myosin heavy chain-1 ❋ Unknown (2009)
Adherent cells were trypsinized and collected in a tube, followed by centrifugation to remove trypsin and medium. ❋ Unknown (2009)
